目的 采用沉香挥发油(essential oil,AEO)、香粉(fragrantpint, AFP)加热及线香(line incense, ALI)燃香熏香吸入给药方式,探究沉香挥发性物质吸入给药镇静促睡眠作用及作用机制。方法 采用对氯苯丙氨酸(PCPA,300 mg·kg-1)诱导的失眠小鼠模型,通过自主活动检测、协同戊巴比妥钠促睡眠行为学试验,考察沉香挥发性物质吸入给药镇静促睡眠的情况,并检测海马神经递质水平及其受体表达情况,探究沉香促睡眠的作用机制。结果 相比模型组,沉香线香(每只0.125 g)、香粉(每只0.1 g)及挥发油(每只1 μL)吸入给药显著抑制小鼠自主活动,延长协同戊巴比妥钠(50 mg·kg-1)促睡眠的时间,有较好镇静促睡眠作用;机制研究结果显示,沉香挥发性物质吸入给药后可不同程度升高γ-氨基丁酸(GABA)、GABA/谷氨酸(Glu)比值、5-羟色胺(5-HT)和腺苷(AD)水平,同时可显著上调Glu合成代谢及转运蛋白的表达水平。结论 沉香挥发性物质吸入给药可较好地镇静促睡眠,其作用机制与调节Glu-GABA平衡及Glu系统合成代谢和转运有关。
Abstract
OBJECTIVE To investigate the sedative and hypnotic effects and mechanism of the inhalation of volatile substances from agarwood by heating volatile oil and powder of agarwood and burning line incense of agarwood. METHODS Insomnia mouse model was induced using chlorobenzene alanine(PCPA, 300 mg·kg-1). Sedation and hypnotic effects were evaluated through autonomic activity detection and collaborative pentobarbital sodium sleep behavior test.The mechanism of agarwood promoting sleep was inspected by detecting the levels of hippocampus neurotransmitters and the expressions of their receptors. RESULTS Compared with model group, agarwood incense(0.125 g/mouse), incense powder(0.1 g/mouse) and volatile oil(1 μL/mouse) inhalation significantly inhibited the spontaneous activity of mice, prolonged the time of sleep promotion of pentobarbital sodium(50 mg·kg-1), and had better sedative and sleep-promoting effects. The results of mechanism study showed that the ratio of γ-aminobutyric acid(GABA)/glutamic acid(Glu) and the levels of GABA, 5-hydroxytryptamine(5-HT) and adenosine were significantly increased, and the expression levels of anabolism and transporter of Glu were significantly up-regulated after inhalation of volatile substances from agarwood. CONCLUSION Inhalation of agarwood volatile substances has sedation and hypnotic effects, and its mechanism is related to the regulation of Glu-GABA balance and the anabolism and transport of Glu system.
关键词
沉香吸入给药 /
失眠 /
镇静 /
催眠 /
神经递质 /
Glu-GABA平衡
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Key words
agarwood inhalation administration /
insomnia /
sedative effect /
hypnotic effect /
neurotransmitter /
Glu-GABA balance
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脚注
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基金
国家自然科学基金青年基金项目资助(82204657);国家重点研发计划项目资助(2018YFC1706400);海南省高层次人才项目资助(2019RC345);海南省重点研发计划项目资助(ZDYF2022SHFZ030)
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