沉香薰香吸入给药镇静促睡眠作用及机制研究

王灿红, 陈细钦, 弓宝, 吴玉兰, 刘洋洋, 魏建和

中国药学杂志 ›› 2022, Vol. 57 ›› Issue (19) : 1628-1635.

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中国药学杂志 ›› 2022, Vol. 57 ›› Issue (19) : 1628-1635. DOI: 10.11669/cpj.2022.19.006
论著

沉香薰香吸入给药镇静促睡眠作用及机制研究

  • 王灿红1, 陈细钦1, 弓宝1, 吴玉兰1, 刘洋洋1*, 魏建和1,2*
作者信息 +

Sedative and Hypnotic Effects of Agarwood Inhalation and Its Mechanism

  • WANG Can-hong1, CHEN Xi-qin1, GONG Bao1, WU Yu-lan1, LIU Yang-yang1*, WEI Jian-he1,2*
Author information +
文章历史 +

摘要

目的 采用沉香挥发油(essential oil,AEO)、香粉(fragrantpint, AFP)加热及线香(line incense, ALI)燃香熏香吸入给药方式,探究沉香挥发性物质吸入给药镇静促睡眠作用及作用机制。方法 采用对氯苯丙氨酸(PCPA,300 mg·kg-1)诱导的失眠小鼠模型,通过自主活动检测、协同戊巴比妥钠促睡眠行为学试验,考察沉香挥发性物质吸入给药镇静促睡眠的情况,并检测海马神经递质水平及其受体表达情况,探究沉香促睡眠的作用机制。结果 相比模型组,沉香线香(每只0.125 g)、香粉(每只0.1 g)及挥发油(每只1 μL)吸入给药显著抑制小鼠自主活动,延长协同戊巴比妥钠(50 mg·kg-1)促睡眠的时间,有较好镇静促睡眠作用;机制研究结果显示,沉香挥发性物质吸入给药后可不同程度升高γ-氨基丁酸(GABA)、GABA/谷氨酸(Glu)比值、5-羟色胺(5-HT)和腺苷(AD)水平,同时可显著上调Glu合成代谢及转运蛋白的表达水平。结论 沉香挥发性物质吸入给药可较好地镇静促睡眠,其作用机制与调节Glu-GABA平衡及Glu系统合成代谢和转运有关。

Abstract

OBJECTIVE To investigate the sedative and hypnotic effects and mechanism of the inhalation of volatile substances from agarwood by heating volatile oil and powder of agarwood and burning line incense of agarwood. METHODS Insomnia mouse model was induced using chlorobenzene alanine(PCPA, 300 mg·kg-1). Sedation and hypnotic effects were evaluated through autonomic activity detection and collaborative pentobarbital sodium sleep behavior test.The mechanism of agarwood promoting sleep was inspected by detecting the levels of hippocampus neurotransmitters and the expressions of their receptors. RESULTS Compared with model group, agarwood incense(0.125 g/mouse), incense powder(0.1 g/mouse) and volatile oil(1 μL/mouse) inhalation significantly inhibited the spontaneous activity of mice, prolonged the time of sleep promotion of pentobarbital sodium(50 mg·kg-1), and had better sedative and sleep-promoting effects. The results of mechanism study showed that the ratio of γ-aminobutyric acid(GABA)/glutamic acid(Glu) and the levels of GABA, 5-hydroxytryptamine(5-HT) and adenosine were significantly increased, and the expression levels of anabolism and transporter of Glu were significantly up-regulated after inhalation of volatile substances from agarwood. CONCLUSION Inhalation of agarwood volatile substances has sedation and hypnotic effects, and its mechanism is related to the regulation of Glu-GABA balance and the anabolism and transport of Glu system.

关键词

沉香吸入给药 / 失眠 / 镇静 / 催眠 / 神经递质 / Glu-GABA平衡

Key words

agarwood inhalation administration / insomnia / sedative effect / hypnotic effect / neurotransmitter / Glu-GABA balance

引用本文

导出引用
王灿红, 陈细钦, 弓宝, 吴玉兰, 刘洋洋, 魏建和. 沉香薰香吸入给药镇静促睡眠作用及机制研究[J]. 中国药学杂志, 2022, 57(19): 1628-1635 https://doi.org/10.11669/cpj.2022.19.006
WANG Can-hong, CHEN Xi-qin, GONG Bao, WU Yu-lan, LIU Yang-yang, WEI Jian-he. Sedative and Hypnotic Effects of Agarwood Inhalation and Its Mechanism[J]. Chinese Pharmaceutical Journal, 2022, 57(19): 1628-1635 https://doi.org/10.11669/cpj.2022.19.006
中图分类号: R965    R285.5   

参考文献

[1] GLIDEWELL R N, MCPHERSON B E, ORR W C. Insomnia and anxiety: diagnostic and management implications of complex interactions[J]. Sleep Med Clin, 2015, 10(1): 93-99.
[2] ROTH T, DRAKE C. Evolution of insomnia: current status and future direction[J]. Sleep Med, 2004, 5(Suppl 1): S23-S30.
[3] DE ZAMBOTTI M, GOLDSTONE A, COLRAIN I M, et al. Insomnia disorder in adolescence: diagnosis, impact, and treatment[J]. Sleep Med Rev, 2018, 39: 12-24.
[4] Ch.P(2015)VolⅠ(中国药典2015年版.一部)[S]. 2015:185.
[5] EDITORIAL BOARD. Herbal Medicines(海药本草)[M]. Vol 5. Shanghai: Shanghai Scientific and Technical Publishers, 1999: 396.
[6] LI S Z. Compendium of Materia Medica(本草原始)[M]. Vol 3. Beijing:People′s Medical Publishing House, 1979:1939.
[7] LI Z L. Primitive Materia Medica(本草从原)[M]. Beijing: Xueyuan Press, 2011.
[8] BAHRAIN H, MOHAMAD J, PAYDAR M J, et al. Isolation and characterisation of acetylcholinesterase inhibitors from Aquilaria subintegra for the treatment of Alzheimer′s disease(AD)[J]. Curr Alzheimer Res, 2014, 11(2): 206-214.
[9] TAKEMOTO H, ITO M, SHIRAKI T, et al. Sedative effects of vapor inhalation of agarwood oil and spikenard extract and identification of their active components[J]. J Nat Med, 2007, 62(1): 41-46.
[10] WANG S, ZHOU Y, MA F L, et al. Effect of agarwood produced by whole-tree agarwood-inducing technique on hypnotic and spontaneous activity inhibition of mice[J]. J Int Pharm Res(国际药学研究杂志), 2016, 43(6): 1082-1087.
[11] ZHANG Y, WANG W, ZHAMG J. Effects of novel anxiolytic 4-butyl-alpha-agarofuran on levels of monoamine neurotransmitters in rats[J]. Eur J Pharmacol, 2004, 504(1): 39-44.
[12] HUO H X, ZHU Z X, PANG D R, et al. Anti-neuroinflammatory sesquiterpenes from Chinese eaglewood[J]. Fitoterapia, 2015, 106: 115-121.
[13] GUO S X. Two new sesquiterpene derivatives from Chinese eaglewood[J]. J Asian Nat Prod Res, 2012, 14(11): 1054-1058.
[14] WANG S, WANG C, PENG D, et al. Agarwood essential oil displays sedative-hypnotic effects through the GABAergic system[J]. Molecules, 2017, 22(12): 2190-2208.
[15] LOPEZ S A, PAGE T. History of use and trade of agarwood[J]. Econ Bot, 2018, 72(1): 107-129.
[16] LIANG Y, KONG D W, ZHOU Q M, et al. Effect of agarwood gas on the sleep of mice by rgulating neurotransmitters[J]. Pharmacol Clin Chin Mater Med(中药药理与临床), 2019, 35(6): 71-77.
[17] TANAKA J, UCHIMURA N, HASHIZUME Y, et al. Effects of aroma on sleep and biological rhythms[J]. Psychiatry Clin Neurosci, 2010, 56(3): 299-300.
[18] LEI L, ZHANG T, GAO D, et al. Study on clinical effect of agarwood fumigation therapy on patients with insomnia disorder[J].J Apoplexy Nerv Dis(中风与神经疾病杂志), 2019, 36(7): 609-612.
[19] SUN Y N, LIANG K, LI Y, et al. Effect of nourishing Yin nourishing blood and anshen prescription on sleep induced by PCPA in mice with insomnia[J]. Chin Arch Tradit Chin Med(中华中医药学刊), 2019, 41(4):178-181.
[20] FAN Y L, ZHANG H, GAN Y, et al. A randomized parallel controlled study on the hypnotic effect of pentobarbital sodium in KM mice[J].Intern Med J Pract Tradit Chin Med Sci(实用中医内科杂志), 2016, 30(9):90-94.
[21] JIAN F Y, LI G X, MA J, et al. Effects of Anshen navel paste on autonomic activity, GABA and 5-HT contents in PCPA-induced insomnia rats[J].World Sci Technol Mod Tradit Chin Med(世界科学技术-中医药现代化), 2017, 19(12): 2012-2016.
[22] WANG G Y, YANG K L, LI D L.The role of 5-HT in the sleep/wake cycle[J]. Chin Contin Med Educ(中国继续医学教育), 2015, 22(1): 1-7.
[23] MU RI GEN JI YA, A GU LA. Effects of Mongolian medicine on the contents of Glu, GABA and Ach in the hippocampus of rats with insomnia[J]. Chin J Ethnomed Ethnopharm(中国民族医药杂志), 2014, 20(6): 43-43.
[24] GUO X. Study on the mechanism of NingxinAnshendecoction in regulating the imbalance of Glu/GABA-Gln metabolic circuit in insomnia rats[D].Beijing: Beijing University of Chinese Medicine, 2017.
[25] TAKATA K, KASAHARA T, KASAHARA M, et al. Erythrocyte/Hep G2-type glucose transporter is concentrated in cells of blood-tissue barriers[J].Biochem Biophys Res Commun, 1990, 173(1): 67-73
[26] YU X Y, SUN Z L. Research progress of Glut1[J].Mod J Integr Tradit Chin West Med(现代中西医结合杂志), 2012, 21(30):3411-3414.
[27] LIU K, KIM J, KIM D W, et al. Lhx6-positive GABA-releasing neurons of the zona incerta promote sleep[J]. Nature, 2017, 548(7669): 582-587.
[28] TAN L B, YU J H, ZHENG X Y, et al. Effect of "ShenningZangxiang" on the behavior and GABA pathway of insomnia rats[J]. World Sci Technol Mod Tradit Chin Med(世界科学技术-中医药现代化),2020, 22(11): 4060-4068.
[29] YAN M Z, CHANG Q, ZHONG Y, et al. Lotus leaf alkaloid extract displays sedative-hypnotic and anxiolytic effects through GABA(A) receptor[J]. J Agric Food Chem, 2015, 63(42): 9277-9285.
[30] QUIONES G M, MAYELI A, YUSHMANOV V E, et al. Reduced GABA/glutamate in the thalamus of individuals at clinical high risk for psychosis[J]. Neuropsychopharmacology,2021,46(6):1133-1139.
[31] REN C Y, CHEN G H, ZHANG M. Progress in regulation of adenosine system on sleep[J]. Chin J Neurol(中华神经科杂志), 2018,51(8): 634-637.
[32] PENG W L, WU Z F, SONG K, et al. Regulation of sleep homeostasis mediator adenosine by basal forebrain glutamatergic neurons[J]. Science, 2020, 369(6508): eabb0556. Doi: 10.1126/science.abb0556.
[33] PENG D Q, YU Z X, WANG C H, et al. Chemical constituents and anti-inflammatory effect of incense smoke from agarwood determined by GC-MS[J]. Int J Anal Chem, 2020, 2020:1-19.Doi:10.1155/2020/4575030

基金

国家自然科学基金青年基金项目资助(82204657);国家重点研发计划项目资助(2018YFC1706400);海南省高层次人才项目资助(2019RC345);海南省重点研发计划项目资助(ZDYF2022SHFZ030)
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